Lab Safety
Recognize, Assess, Minimize, and Prepare for Lab Hazards
There are a variety of hazards that may be found in a laboratory. Know the hazards of the materials you will work with. Read lab safety and experiment procedures, chemical SDS and agent biosafety summaries/practices before starting. Safety is everyone's responsibility. Don't allow yourself to become complacent over time. Consider others and maintain a clean working environment. Please email us at EHS@uhcl.edu if you need assistance with resources or information on requirements.
Lab Safety Procedures, Reference Information
Chemical Hygiene Plan (CHP) (replaces Lab Safety Manual)
- High Risk Chemical Review Form (CHP App. 6)
- High Risk Chemicals Requiring Protocol Review (CHP App. 5)
- Chemical SOP Template (editable)
- Graduate Student Request to Work Independently After Hours
Hazard Communication Plan (pp. 64-71 of CHP)
Pregnant Lab Workers - Declaration Letter and Procedures
Research Applications
Student Researchers Policy & Procedures
- Student Researcher Application
- Release of Indemnification form
- Graduate Student Request to Work Independently After Hours
Minors in the Lab Policy & Procedures
(for non-UHCL students, including 12th grade)
Visiting Researchers Policy & Procedures
Laboratory and/or Equipment Checkout Procedures
Chemical Labels
All chemical containers must be labeled with the original manufacturer's label, or:
- Replacement Label if the original container label gets damaged or becomes illegible.
- Secondary Container Label filled out with chemical name and hazards checked to meet Hazard Communication requirements.
- Small Containers or Samples – can have one or combination of:
- A label hanging on the container or attached using tape;
- Logbook visible in the lab nearby with chemical name and hazards, when only sample number may fit on the container;
- Acronyms list posted in the lab nearby with chemical name and hazards, when only acronym may fit on the container.
Chemical Labels & Labeling - Replacing original labels, labeling secondary containers (prepared solutions, samples), and Wastes. See also Chemical Safety dropdown below for label Hazard Information.
Not Food or Drink Labels - Print on Avery 5160 return address labels and place on consumable items used for lab experiments.
Glassware
- Glass Handling and Disposal - Intact or Broken
- Controlled Glassware and Precursor Chemicals
Inspections
- General Lab Inspection Checklist
- Lab Safety Inspection Checklist in EHSA Software
Inspections are done within the EHSA software, with notifications emailed to responsible individuals. Corrective actions are done by and entered into EHSA software by responsible individuals according to the inspection procedures outlined in the Chemical Hygiene Plan.
References
Safety in Academic Chemistry Laboratories - American Chemical Society publication
The Lab Safety Institute has compiled over 5,000 examples of accidents in labs for over 40 years. They have published these examples for purchase in bulk, or as a summary. Here is the index of other accident examples, and some of the worst in their 100 Years of Progress. Please contact x.2106 for access.
Lab Safety Training
See the Laboratory Training Matrix for what is required to conduct work in a UHCL lab.
- Student Lab Safety Training Policy
- Advanced Lab Safety Training Policy for TA's, RA's, Graduate Independent Studies, Lab Faculty and Staff
Training Enrollment
Students taking laboratory courses utilizing chemicals are enrolled at the beginning of every semester in Lab Safety Training within Canvas. Late registering students must email coen@uhcl.edu for enrollment.
A separate Advanced Lab Safety Training course is given to TA's, RA's, Graduate Independent Studies, and Lab Faculty and Staff.
Additional lab specific trainings are available as modules within the Canvas Lab Safety Training Topics course.
Training Courses
These training presentations may be taken in Canvas. See the Lab Training Matrix for a list of what training and frequency is required for your lab.
- Lab and Chemical Safety Training - including Hazard Communication, fire and emergencies, first aid, bloodborne pathogens awareness
- Safety in Academic Chemistry Laboratories - American Chemical Society Publication (resource)
- Chemical Labels and Labeling - Relabeling original containers, labeling Secondary containers (prepared solutions, samples), Waste labels
- Fume Hoods User Training
- Waste handling, Collection & Labeling
- Autoclave, Disinfection, and Blood Borne Pathogens Training
Videos
- Lab Safety Institute Favorites
- Chemical Safety Board Videos- search for Experimenting with Danger, and After the Rainbow
Humorous Videos
Personal Protective Equipment (PPE)
PPE may be purchased at the bookstore. If purchased elsewhere, it must match the same protection as those chosen for sale at the bookstore. i.e., same length lab coat down legs and long sleeves, same size and design safety glasses and goggles with indirect vents and Z87 impact rating.
Lab Coats
Lab coats must be full length to the knees with long sleeves. Dental coats or doctors coats do not provide sufficient coverage. A blend of polyester and cotton is recommended to retain both liquid and flame retardant properties. 100% polyester lab coats shall not be used with flammable or pyrophoric materials. 100% cotton absorbs chemicals more easily and burns hotter in a fire if they do catch fire.
Safety goggles
Safety goggles with indirect vents are required for use with liquids, as the risk of splash and drip into the eye is present. Safety goggles should fit the face well to provide a seal. Safety glasses may be used with dry materials, and are chosen to provide the most coverage.
Gloves
Gloves are not suitable for use with every chemical. There are many types of glove materials. Each type is compatible and incompatible with different materials. Search by chemical for the recommended glove type, and then by the manufacturer for breakthrough time (length of time use) for that glove thickness.
- Example: Best Brand N-Dex regular disposable gloves are not suitable for concentrated sulfuric acid. They have a breakthrough time of 10 minutes or less.
- Some Different Glove Types and Uses
- Glove Selection by Chemical Search-Cole Parmer
- Chemical Resistance Guide - Permeation & Degradation Data
Chemical Inventory
The UHCL Chemical Inventory is compiled within the OnSite Environmental Health & Safety Assistant (EHSA) software. Chemical inventory is required by law annually, and serves several functions:
- To know what materials are present and assess their hazards
- A list of SDS needed, and repository to store SDS for reviewing hazards of chemicals used, hazard planning, training and first aid purposes
- Use for several agency reports (e.g., DEA, TCEQ, DSHS)
- Allow faculty to share or dispose of items.
- Chemical Inventory Template for Import into OnSite
- Requirement and Completion information
- Notice to Employees, and en español
Chemical Safety
Chemical Hygiene Plan 2024 (replaces Lab Safety Manual)
Safety Data Sheets (SDS)
Login with your UHCL credentials to Search for Safety Data Sheets (SDS) on the chemicals you will work with: https://onsite.uhcl.edu/ehsa/login?/showSdsHubSearch
Go to the Safety Data Sheets (SDS) page for additional information, and manufacturer's links to obtain SDS for your lab or chemical inventory.
Hazard Communication
Chemical Labels
All chemical containers must be labeled with the original manufacturer's label, or:
- Replacement Label if the original container label gets damaged or becomes illegible.
- Secondary Container Label filled out with chemical name and hazards checked to meet Hazard Communication requirements.
- Small Containers or Samples – can have one or combination of:
- A label hanging on the container or attached using tape;
- Logbook visible in the lab nearby with chemical name and hazards, when only sample number may fit on the container;
- Acronyms list posted in the lab nearby with chemical name and hazards, when only acronym may fit on the container.
Chemical Labels & Labeling - Replacing original labels, labeling secondary containers (prepared solutions, samples), and Wastes.
Not Food or Drink Labels - Print on Avery 5160 return address labels and place on consumable items used for lab experiments.
Hazard Communication Plan (pp. 64-71 of CHP)
- HazCom-GHS Training (for facilities, reference) - current version contained in Lab Safety Training
Hazard Information
- Chemical Labels 2015+ (Ingles y en Espanol)
- Chemical Labels 2015+ & prior NFPA diamond hazards
- Pictograms & Hazards List (Ingles y en Espanol)
- Safety Data Sheet (SDS) chemical information fields
- Ficha de datos de seguridad (SDS) campos de información química
- Chemical Inventory requirement
Chemical Compatibility and Storage
- Chemical Storage - Segregation Groups (one page Pictoral)
- Chemical Segregation and Compatibility (CHP pp.57-63)
- Berkeley Segregation and Compatibility
- Classes of INCOMPATIBLE Materials
- DOT Segregation Table (used for transportation safety)
- EPA Chemical Compatibility Chart
High Hazard Materials
- PYROPHORIC Chemicals – DOE Handbook
- PYROPHORIC Materials – University of Berkeley
- WATER REACTIVE Chemicals – University of Berkeley
Peroxide Forming, Potentially Explosive Chemicals (PEC's)
- Peroxide Forming Chemicals List & Testing Frequency
- Peroxide Forming Chemicals Labels
- Peroxide Forming Storage Shelf Sign
- Perchloric Acid Hazards - University of Maryland, Cornell
All peroxide forming containers should have a peroxide label on them. Labels and test strips are in the stockroom. The storage area sticker should be placed above the shelf where peroxide formers are stored, in a flammable cabinet.
Testing for peroxides is required, often annually, and before distilling or concentrating. Failure to test and manage inventory can lead to a potentially dangerous explosive situation, and require dedicated costly high hazard stabilization waste disposal.
Old, unknown age, poor condition, or crystallized peroxide formers (inside bottle or under cap) should not be opened or moved as they may pose an explosion risk. Notify Hank Grotewold@uhcl.edu for those high hazard waste pickup needs.
HydroFluoric Acid
- HydroFluoric Acid Safety - University of Delaware
- HydroFluoric Acid SOP - Mount Sinai
- Spill, Splash Quick Guide for HF exposure
- Medical Treatment Guide for HF exposure
Biological Safety
- UH-Biological Safety Manual
- UHCL Bloodborne Pathogens Exposure Control Plan
- UHCL Autoclave, Disinfection, and Blood Borne Pathogens Training for Labs (taken in blackboard, Employee EHS training)
- Autoclave Procedures
- Autoclave Log and Efficacy Testing Sheets
- Autoclave Bag Tags
See below for Biological Safety Cabinet and Fume Hood use information.
Incident Reporting
All incidents and injuries on campus or University business must be reported within 24 hours to EHS@UHCL.edu, and HumanResources@uhcl.edu as well for employee reports.
If you witness an incident, a witness statement must be submitted.
Go to the Incident Reporting Forms section on the EHS Forms page for fillable pdf forms for employees, students and visitors, and witnesses.
Safety Concern, Near Miss & Equipment Incident Report Form
In the same way accidents/incidents are investigated for actions to prevent recurrence, it is important to learn from near misses and eliminate unsafe conditions to prevent an accident from actually occurring.
UHCL asks that all employees report and correct potential hazards concerns and near misses immediately to help prevent incidents and maintain a safe work environment. Incidents that result in damage to equipment must also be reported within 24 hours. Submit these reports to EHS@uhcl.edu.
(Accident theory model illustration by Heinrich & Bird)
Environmental Health and Safety Assistant (EHSA) Software
UHCL uses this software to keep track of regulatory obligations, chemical inventories, SDS, etc. Login is the same as your UHCL user id and password. There is also a searchable database hub for SDS.
Fume Hoods, Biosafety Cabinets
Labs may be equipped with chemical fume hood(s) and/or biological safety cabinets. Here is information on safe work practices, how they work, and a decision matrix for selecting the right one.
Chemical Fume Hoods
- Fume Hoods User Guide training
- User Information Posted on fume hoods
- Fume Hood diagram and Safety Reminders
- Fume Hood Containment illustrations
Biological Safety Cabinets
Waste
- Lab Waste Handling Summary Chart - Overview of all lab wastes
- Chemical & Hazardous Wastes
Waste Labeling
Waste Documentation
Glassware
- Broken Glass box sign
- Glass Handling and Disposal - Intact or Broken
- Controlled Glassware and Precursor Chemicals
High Hazard Waste
- Peroxide Forming Chemicals Testing & Disposal Chart
- Peroxide Forming Chemical Labels
- Peroxide Storage Area Label
Waste Training
- Lab Waste Training (taken in Canvas or in person)
See Biological Safety section for biological waste and autoclaving procedures.
Waste Pickups
Wastes are picked up at least twice a year from designated lab waste storage areas (in each building, not each lab). Lab waste containers (<5 gal size) should be brought to the designated waste storage area within 3 days when full. Contact Hank Grotewold@uhcl.edu for questions or assistance.
Laser Safety
All class 3b and 4 lasers must be approved and registered with the state. New laser proposals, locations, and authorized users must be requested by sub-registration application or amendment to the Laser Safety Officer (LSO) x.2107 to obtain prior authorization and training.
- Notice to Employees: Texas Regulations for Control of Radiation - Lasers (PDF)
- Laser Safety Awareness & Refresher Training - for guests and visitors (taken in Blackboard, Employee EHS training shell)
- Laser Safety Awareness Simple Training - for visitors
- UHCL Certificate of Registration for Lasers - License with the State
- Laser Safety Manual UHCL
- Laser Sub-registration Amendment form - to request new lasers or users
Laser Regulations and Requirements
Radiation Safety
All radioactive source materials, locations, authorized users, and equipment containing sources must be approved and registered with the state on our license.
Any changes in radiation source, use, equipment, locations, or users must be requested by Amendment application Form (below) to the Radiation Safety Officer (RSO) x.2107 for requirements review (training, procedures, setup) and subsequent RSO submission to the state (for most changes) to receive permission for an amendment to the present radiation license, prior to activities. Expect at least a month turnaround time.
New activities, users, and equipment, PI's may petition via application below. Expect longer turnaround time for review, controls, and application amendment with the RSO and state.
- Notice to Employees: Texas Regulations for Control of Radiation
- Application for PI/Isotope/Equipment (new PI sub-license)
- Amendment Form (to update users, isotope, equipment use)
- RSO Duties and Responsibilities
- UH Radiation Safety Enforcement reference
Radioisotope Regulations
- Texas Health and Human Services: Laws and Rules - Radioactive Materials Licensing
- 25 TAC 289.252 Licensing of Radioactive Material
- 25 TAC 289.228 Radiation Safety Requirements for Analytical Machines
- 25 TAC 289.231 General Provisions and Standards for Protection Against Machine Produced Radiation
- 25 TAC 289.255 Radiation Safety Requirements for sources of radiation used in machines
X-Ray Safety Program
All equipment producing x-rays must be approved and registered with the state. New equipment proposals and new authorized users must be requested by sub-registration application or amendment to the X-ray Radiation Safety Officer (XRSO) x.2107 to obtain prior authorization and training.
- X-Ray Radiation Safety Manual
- X-Ray Equipment Application (sub-registration to request new equipment)
- X-Ray Equipment Amendment Form (to update users or equipment use)
- UHCL Certificate of Registration for X-Ray Registration - License with the State
- Notice to Employees: Texas Regulations for Control of Radiation (PDF)
X-Ray Regulations
- Texas Health and Human Services: Laws and Rules - X-Ray Machines and Services
- 25 TAC 289.226 Registration of Radiation Machine Use and Services
- 25 TAC 289.227 Use of Radiation Machines in the Healing Arts
- 25 TAC 289.228 Radiation Safety Requirements for Analytical Machines
- 25 TAC 289.231 General Provisions and Standards for Protection Against Machine Produced Radiation